LOCUS       sequence-149560-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCherry-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..696
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
       61 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      121 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      181 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      241 ggcatggacg agctgtacaa gggatccttg gctactacac ttgaacgtat tgagaagaac
      301 tttgtcatta ctgacccaag attgccagat aatcccatta tattcgcgtc cgatagtttc
      361 ttgcagttga cagaatatag ccgtgaagaa attttgggaa gaaactgcag gtttctacaa
      421 ggtcctgaaa ctgatcgcgc gacagtgaga aaaattagag atgccataga taaccaaaca
      481 gaggtcactg ttcagctgat taattataca aagagtggta aaaagttctg gaacctcttt
      541 cacttgcagc ctatgcgaga tcagaaggga gatgtccagt actttattgg ggttcagttg
      601 gatggaactg agcatgtccg agatgctgcc gagagagagg gagtcatgct gattaagaaa
      661 actgcagaaa atattgatga ggcggcaaaa gaacttatca agtgtgtggt ggtgggagac
      721 ggagctgtag gtaaaacttg cctactgatc agttacacaa ccaatgcatt tcctggagaa
      781 tatatcccta ctgtctttga caattattct gccaatgtta tggtagatgg aaaaccggtg
      841 aatctgggct tatgggatac agctggacta gaagattatg acagattacg ccccctatcc
      901 tatccgcaaa cagatgtgtt cttaatttgc ttttcccttg tgagtcctgc atcattccac
      961 cacgtccgtg caaagtggta tcctgaggtg cggcaccact gtcccaacac tcccatcatc
     1021 ctagtgggaa ctaaacttga tc
//
