LOCUS       sequence-149572-        3841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rluc8 reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pTRE-Tight-Rluc8
FEATURES             Location/Qualifiers
     source          1..3841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..324
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     297..321
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    1434..1515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1471..1490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1618..1640
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1678..1696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1764..1868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1869..1937,1938..2729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2087..2106)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2900..3488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3389..3408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3642..3659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3776..3797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3812..3817,3818..3835,3836..3841,1)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gtgtggctcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
       61 cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      121 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      181 cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      241 gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      301 cgtttagtga accgtcagat cgcctggaga attcgagctc ggtacccggg gatccaccat
      361 gaaggtgtac gaccccgagc agaggaagag gatgatcacc ggcccccagt ggtgggccag
      421 gtgcaagcag atgaacgtgc tggacagctt catcaactac tacgacagcg agaagcacgc
      481 cgagaacgcc gtgatcttcc tgcacggcaa cgccactagc agctacctgt ggaggcacgt
      541 ggtgccccac atcgagcccg tggccaggtg catcatcccc gatctgatcg gcatgggcaa
      601 gagcggcaag agcggcaacg gcagctacag gctgctggac cactacaagt acctgaccgc
      661 ctggttcgag cttctgaacc tgcccaagaa gatcatcttc gtgggccacg actggggcgc
      721 cgccctggcc ttccactacg cctacgagca ccaggacagg atcaaggcca tcgtgcacat
      781 ggagagcgtg gtggacgtga tcgagagctg ggacgagtgg ccagacatcg aggaggacat
      841 cgccctgatc aagagcgagg agggcgagaa gatggtgctg gagaacaact tcttcgtgga
      901 gaccgtgctg cccagcaaga tcatgagaaa gctggagccc gaggagttcg ccgcctacct
      961 ggagcccttc aaggagaagg gcgaggtgag aagacccacc ctgagctggc ccagagagat
     1021 ccccctggtg aagggcggca agcccgacgt ggtgcagatc gtgagaaact acaacgccta
     1081 cctgagagcc agcgacgacc tgcccaagct gttcatcgag ggcgaccccg gcttcttcag
     1141 caacgccatc gtggagggcg ccaagaagtt ccccaacacc gagttcgtga aggtgaaggg
     1201 cctgcacttc ctccaggagg acgcccccga cgagatgggc aagtacatca agagcttcgt
     1261 ggagagagtg ctgaagaacg agcagtaagc ggccgcatcg ataagcttgt cgacgatatc
     1321 tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1381 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1501 catttttttc actgcctcga gagttaagcc agccccgaca cccgccaaca cccgctgacg
     1561 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     1621 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     1681 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3661 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3721 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3781 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     3841 t
//
