LOCUS       sequence-149573-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-zeoCV
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    28..109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     53..72
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(123..497)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(516..563)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(660..684)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(681..780)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(710..730)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccgggttaat taaggcgcgc cagatctgca gtgaaaaaaa tgctttattt gtgaaatttg
       61 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttt cgaggtcgag
      121 tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg
      181 aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg
      241 aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacacac
      301 acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc
      361 acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc
      421 cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca
      481 ctggtcaact tggccatggt ttagttcctc accttgtcgt attatactat gccgatatac
      541 tatgccgatg attaattgtc aacacggtcc gttccaatgc accgttcccg gccgcggagg
      601 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
      661 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
      721 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
      781 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
      841 aaccgctatc cacgcccatt gatgtactgc ca
//
