LOCUS       sequence-149575-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-zeoCV
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..103
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             122..496
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    510..591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(547..566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(665..683)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(666..683)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(764..783)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgg accgtgttga
       61 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
      121 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
      181 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
      241 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
      301 caacaccctg gcctgggtgt gtgtgcgcgg cctggacgag ctgtacgccg agtggtcgga
      361 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
      421 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
      481 cgaggagcag gactgacact cgacctcgaa acttgtttat tgcagcttat aatggttaca
      541 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cagatctggc
      601 gcgccttaat taacccgggg atccgtcgac ctgcagcgta cgaagcttca gctggcggcc
      661 gcgttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
      721 agccgcgttc taacgacaat atgtccatat ggtgcactct cagtacaatc tgctctgatg
      781 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      841 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      901 a
//
