LOCUS       sequence-149581-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b-PK6
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..84
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             88..129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             130..171
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             178..219
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             223..264
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             265..306
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      336..523
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(391..415)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        582..925
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacgcta gcggtaagcc tattcctaac
       61 cctcttttgg gtctcgattc tacaggtgga aaaccaatcc ccaacccact cctcggcctt
      121 gactcaactg gcaaacccat accaaatcct ttacttggat tagatagtac ctctagcggt
      181 aagcctattc ctaaccctct tttgggtctc gattctacag gtggaaaacc aatccccaac
      241 ccactcctcg gccttgactc aactggcaaa cccataccaa atcctttact tggattagat
      301 agtacctcta gatgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
      361 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
      421 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
      481 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctaggagat
      541 ctgtttagct tgcctcgtcc ccgccgggtc acccggtcag cgacatggag gcccagaata
      601 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
      661 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
      721 cgcgaagcaa aaattacggc tcctcgctgc ggacctgcga gcagggaaac gctcccctca
      781 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
      841 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
      901 acatcacatc cgaacataaa caaccatggg taaaaagcct gaactcaccg cgacgtctgt
      961 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcgga
//
