LOCUS       sequence-149584-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of 
            the SunTag system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(356..376)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(452..471)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttctccgctg tcgaacagca gggcgccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtcca
      301 tggtggcgat cccgggcccg cggtaccgca agcttgatat cctgcagacg cgtcccacct
      361 tgcgcttctt cttgggcatg gtggcgcgcg tagcctaggc ctccaaaaaa gcctcctcac
      421 tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt
      481 agtcagccat ggggcgga
//
