LOCUS       sequence-149586-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b-PK9
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..85
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             89..130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             131..172
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             179..220
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             224..265
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             266..307
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             314..355
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             359..400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             401..442
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      472..659
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(527..551)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcggtaagc ctattcctaa
       61 ccctcttttg ggtctcgatt ctacaggtgg aaaaccaatc cccaacccac tcctcggcct
      121 tgactcaact ggcaaaccca taccaaatcc tttacttgga ttagatagta cctctagcgg
      181 taagcctatt cctaaccctc ttttgggtct cgattctaca ggtggaaaac caatccccaa
      241 cccactcctc ggccttgact caactggcaa acccatacca aatcctttac ttggattaga
      301 tagtacctct agcggtaagc ctattcctaa ccctcttttg ggtctcgatt ctacaggtgg
      361 aaaaccaatc cccaacccac tcctcggcct tgactcaact ggcaaaccca taccaaatcc
      421 tttacttgga ttagatagta cctctagatg aggcgcgcca cttctaaata agcgaatttc
      481 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt
      541 taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag
      601 gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc
      661 agatccgcta ggagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggtcagcgac
      721 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
      781 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
      841 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcggac ctgcgagcag
      901 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
      961 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     1021 ctagga
//
