LOCUS       sequence-149588-       10161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pKA-207I10
FEATURES             Location/Qualifiers
     source          1..10161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2136..2145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2295..2528
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    2532..2588
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(2590..3164,3165..3167,3168..3176)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2757..2774)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2984..3003
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5424..6131
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5569..5587)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5837..5856
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6204..6827
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MTRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(6277..6296)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    6949..7070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6986..7005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7040..7059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7077..7532)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7214..7235)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7426..7445
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7559..7663
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7665..8022
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7665..7856
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7688..7708)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7873..8008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8057..8851
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8057..8851
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8111..8130)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8721..8740
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9039..9058)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9083..9130
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9459..10047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9948..9967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 tgtcgcggag gcggcacagc tacgagaacg atggtggaca acctcacaaa aggaggaaga
      661 cgtctccacc ggtggcgtca aagtccgttc atgccgacat cacccttctg ctcgatatgg
      721 agggtgtgat tcgcgaagcc accctgtctc cgacgatggc ggccgagagc gtggacggtt
      781 ggctggggcg tcgctggagc gacatcgccg gcgccgaagg cggcgacaag gttcgccgca
      841 tggtcgaaga cgcccgccgc agcggcatct cggctttccg ccagatcaat cagcctttcc
      901 cgagcggcgt cgaaatcccg atcgaattca ccacgatgct gctgggcgac cgcaccggca
      961 tgatcgcggt cggcaagaac atgcaggcgg tcaccgagct gcattcccgg ctgatcgctg
     1021 cgcagcaggc gatggagcgc gactattggc ggttgcgtga attggagact cgctaccgcc
     1081 tggtgttcga cgctgccgcc gatgcggtga tgatcgtctc cgccggcgac atgcgcatcg
     1141 tcgaagccaa ccgggcggcg gtgaatgcga tcagccgcgt cgagcgcggc aatgacgacc
     1201 ttgcggggcg tgatttcctc gccgaagtgg cggctgccga tcgcgatgcg gtgcgcgaca
     1261 tgctggccca ggtgcgtcag cgcggcaccg cactcagcgt cctcgttcat ctcggccgtt
     1321 acgaccgcgc ctggatgctg cgcggttcgc tgatgtcgtc cgagcgtcgt caggttttcc
     1381 tgctgcactt caccccggtg accacgactc ccgcgatcga cgacgtcgac gatgatgccg
     1441 tgctgcgcgg gctgatcgat cgcattcccg acgggttcgt cgcactggat tcggaaggcg
     1501 tcgttcgtca cgccaaccag gcgtttctcg atctggtcca gatcggctcc aagcctgcgg
     1561 cggtcggacg atcgctgggc gtctggatgg gtcgtccggg cgccgatctg tccagcttgc
     1621 tgacgctgct gcggcgctac aagacggtgc ggctgttcca aacgacgatc cgcggcgagc
     1681 tcggcaccga gactgaagtc gaggtctcgg ccgtcgacgg cgaggacgac caatacatcg
     1741 gcgttctgat gcgcaatgtc gcgcgacgcc tcgacgctgc ggacgaccac gatgccttgc
     1801 gtcaggcgct cggcccgatc agcaagcagc tcgggcgatc ctcgctgcgc aagctggtga
     1861 agaacgccgt gagcattgtc gagcagcact acgtgaagga agcgctgttg cgatccaagg
     1921 gcaatcgcac ggcaactgcc gaactgctcg gattgagccg gcagagcctt tatgcaaaac
     1981 tcaacagcta cggcttcgac gacaaaggtg tcgttgcttc tgctgccgac ggtgcagagg
     2041 gcgcctcaga cgacgcagag gataagctta gtgctggtgg tagtgctggt ggtagtgctg
     2101 gtggtagtgc tggtggtagt gctggtggac cggtcgccac catggcgtac agccgcgcgc
     2161 gtacgaaaaa caattacggg tctaccatcg agggcctgct cgatctcccg gacgacgacg
     2221 cccccgaaga ggcggggctg gcggctccgc gcctgtcctt tctccccgcg ggacacacgc
     2281 gcagactgtc gacggccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg
     2341 gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg
     2401 gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc
     2461 tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt
     2521 acggtgggta aagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     2581 cgggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     2641 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2701 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2761 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2821 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2881 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2941 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3001 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3061 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     3121 gaaccacggg gacgtggttt tcctttgaaa aacacggtga taatgtggcc acaaccatgg
     3181 tgaccggtgt ggcaggtcat gcctctggca gccccgcatt cgggaccgcc gatctttcga
     3241 attgcgaacg tgaagagatc cacctcgccg gctcgatcca gccgcatggc gcgcttctgg
     3301 tcgtcagcga gccggatcat cgcatcatcc aggccagcgc caacgccgcg gaatttctga
     3361 atctcggaag cgtgctcggc gttccgctcg ccgagatcga cggcgatctg ttgatcaaga
     3421 tcctgccgca tctcgatccc accgccgaag gcatgccggt cgcggtgcgc tgccggatcg
     3481 gcaatccctc cacggagtac gacggtctga tgcatcggcc tccggaaggc gggctgatca
     3541 tcgagctcga acgtgccggc ccgccgatcg atctgtccgg cacgctggcg ccggcgctgg
     3601 agcggatccg cacggcgggc tcgctgcgcg cgctgtgcga tgacaccgcg ctgctgtttc
     3661 agcagtgcac cggctacgac cgggtgatgg tgtatcgctt cgacgagcag ggccacggcg
     3721 aagtgttctc cgagcgccac gtgcccgggc tcgaatccta tttcggcaac cgctatccgt
     3781 cgtcggacat tccgcagatg gcgcggcggc tgtacgagcg gcagcgcgtc cgcgtgctgg
     3841 tcgacgtcag ctatcagccg gtgccgctgg agccgcggct gtcgccgctg accgggcgcg
     3901 atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc gatccatctg cagtacctga
     3961 agaacatggg cgtgcgcgcc accctggtgg tgtcgctggt ggtcggcggc aagctgtggg
     4021 gcctggttgc ctgtcaccat tatctgccgc gcttcatcca tttcgagctg cgggcgatct
     4081 gcgaactgct cgccgaagcg atcgcgacgc ggatcaccgc gcttgagagc ttcgcgcaga
     4141 gccagtcgga gctgttcgtg cagcggctcg aacagcgcat gatcgaagcg atcacccgtg
     4201 aaggcgattg gcgcgcagcg attttcgaca ccagccaatc gatcctgcag ccgctgcacg
     4261 ccgacggttg cgcgctggtg tacgaagacc agatcaggac catcggtgac gtaccttcca
     4321 cgcaggatgt tcgcgagatc gccgggtggc tcgatcgcca gccacgtgcg gcggtgacct
     4381 cgaccgcgtc gctcggtctc gacgtgccgg agctcgcgca tctgacgcgg atggcgagcg
     4441 gcgtggtcgc ggcgccgatt tcggatcatc gcggcgagtt tttgatgtgg ttccgccccg
     4501 agcgcgtcca caccgttacc tggggcggcg atctgaagaa gccgttcacg atgggcgata
     4561 caccggcgga tctgtcgccg cggcgctcct tcgccaaatg gcatcaggtt gtcgaaggca
     4621 cgtccgatcc gtggacggcc gccgatctcg ccgcggctcg caccatcggt cagaccgtcg
     4681 ccgacatcgt gctgcaattc cgcgcggtgc ggacactgat cgcccgcgaa cagtacgaac
     4741 agttttcgtc ccaggtgcac gcttcgatgc agccggtgct gatcaccgac gccgaaggcc
     4801 gcatcctgct gatgaacgac tcgttccgcg acatgttgcc ggcggggtcg ccatccgccg
     4861 tccatctcga cgatctcgcc gggttcttcg tcgaatcgaa cgatttcctg cgcaacgtcg
     4921 ccgaactgat cgatcacggc cgcgggtggc gcggcgaagt tctgctgcgc ggcgcaggta
     4981 atcgcccgtt gccgctggca gtgcgcgccg atccggtgac gcgcacggag gaccagtcgc
     5041 tcggcttcgt gctgatcttc agcgacgcta ccgatcgtcg caccgcagat gccgcacgca
     5101 cgcgtttcca ggaaggcatt cttgccagcg cacgtcccgg cgtgcggctc gactccaagt
     5161 ccgacctctt gcacgagaag ctgctgtccg cgctggtcga gaacgcgcag cttgccgcat
     5221 tggaaattac ttacggcgtc gagaccggac gcatcgccga gctgctcgaa ggcgttcgcc
     5281 agtcgatgct gcgcaccgcc gaagtgctcg gccatctggt gcagcacgcg gcgcgcacgg
     5341 ccggcagcga cagctcgagc aatggctcgc agaacaagaa ggaattcgat agtgctggta
     5401 gtgctggtag tgctggtact agtatggtga gcaagggcga ggaggataac atggccatca
     5461 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     5521 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     5581 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     5641 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     5701 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     5761 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     5821 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     5881 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     5941 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     6001 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     6061 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     6121 agctgtacaa gagtgctggt gggtccgccg gcgggagtgc tggcggtagt gccggcggta
     6181 gttctagaag tgctggtggg tccatgacta gattagataa aagtaaagtg attaacagcg
     6241 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     6301 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
     6361 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     6421 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     6481 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     6541 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
     6601 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     6661 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     6721 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     6781 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcctaagcg gccgcgactc
     6841 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     6901 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     6961 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7021 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     7081 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7141 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     7201 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     7261 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7321 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7381 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7441 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7501 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     7561 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7621 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     7681 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7741 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7801 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7861 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7921 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7981 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     8041 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     8101 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     8161 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     8221 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     8281 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     8341 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     8401 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     8461 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     8521 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     8581 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     8641 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     8701 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     8761 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8821 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     8881 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8941 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9001 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     9061 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     9121 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     9181 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     9241 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     9301 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9601 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10081 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10141 aaccgtatta ccgccatgca t
//
