LOCUS       sequence-149591-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b-PK12
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..88
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             92..133
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             134..175
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             182..223
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             227..268
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             269..310
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             317..358
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             362..403
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             404..445
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             452..493
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             497..538
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             539..580
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      610..797
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(665..689)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ttcgtacgct gcaggtcgac ggatccccgg gttaattaac gctagcggta agcctattcc
       61 taaccctctt ttgggtctcg attctacagg tggaaaacca atccccaacc cactcctcgg
      121 ccttgactca actggcaaac ccataccaaa tcctttactt ggattagata gtacctctag
      181 cggtaagcct attcctaacc ctcttttggg tctcgattct acaggtggaa aaccaatccc
      241 caacccactc ctcggccttg actcaactgg caaacccata ccaaatcctt tacttggatt
      301 agatagtacc tctagcggta agcctattcc taaccctctt ttgggtctcg attctacagg
      361 tggaaaacca atccccaacc cactcctcgg ccttgactca actggcaaac ccataccaaa
      421 tcctttactt ggattagata gtacctctag cggtaagcct attcctaacc ctcttttggg
      481 tctcgattct acaggtggaa aaccaatccc caacccactc ctcggccttg actcaactgg
      541 caaacccata ccaaatcctt tacttggatt agatagtacc tctagatgag gcgcgccact
      601 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
      661 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
      721 agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt
      781 gaccacacct ctaccggcag atccgctagg agatctgttt agcttgcctc gtccccgccg
      841 ggtcacccgg tcagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
      901 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
      961 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
     1021 ctgc
//
