LOCUS       sequence-149595-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b-HA3
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..69
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             73..99
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      165..352
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(220..244)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        411..754
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgctagca tgtacccata cgatgttcct
       61 gactatgcgg gctacccgta tgacgtgccg gactatgcag gagcctatcc ttatgacgtt
      121 ccagattacg ctggttctag atgaggcgcg ccacttctaa ataagcgaat ttcttatgat
      181 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
      241 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
      301 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
      361 ctaggagatc tgtttagctt gcctcgtccc cgccgggtca cccggtcagc gacatggagg
      421 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      481 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      541 gccgcacggc gcgaagcaaa aattacggct cctcgctgcg gacctgcgag cagggaaacg
      601 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      661 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      721 acagttctca catcacatcc gaacataaac aaccatgggt aaaaagcctg aactcaccgc
      781 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
      841 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
      901 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
      961 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     1021 ctattgcatc tcccgc
//
