LOCUS       sequence-149636-        2302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor. Visualization of FAK activity at focal adhesions in 
            live cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Seong J, Tajik A, Sun J, Guan JL, Humphries MJ, Craig SE, Shekaran 
            A, Garcia AJ, Lu S, Lin MZ, Wang N, Wang Y
  TITLE     Distinct biophysical mechanisms of focal adhesion kinase 
            mechanoactivation by different extracellular matrix proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19372-7. doi: 
            10.1073/pnas.1307405110. Epub 2013 Nov 12.
  PUBMED    24222685
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1307405110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-11-26- 26"; volume: "110"; issue: "48"; pages: "19372-7"
COMMENT     Sequence Label: FAT FAK biosensor
FEATURES             Location/Qualifiers
     source          1..2302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1088..1798
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
ORIGIN
        1 ggatcccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc ccgcatgcat tggtattttg ggaagatcac
      721 tcgtcgggag tccgagcggc tgctgctcaa ccccgaaaac ccccggggaa ccttcttggt
      781 ccgggagagc gagacgacaa aaggtgccta ttgcctctcc gtttctgact ttgacaacgc
      841 caaggggctc aatgtgaagc actacaagat ccgcaagctg gacagcggcg gcttctacat
      901 cacctcacgc acacagttca gcagcctgca gcagctggtg gcctactact ccaaacatgc
      961 tgatggcttg tgccaccgcc tgactaacgt ctgtgggtct acatctggat ctgggaagcc
     1021 gggttctggt gagggttctg agaccgacga ctacgctgag atcatcgacg aggaggagct
     1081 cgagctctct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     1141 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctacgta
     1201 cggtaaattg accttaaaat tactctgtac tactggtaaa ttgccagttc catggccaac
     1261 cttagtcact actttaggtt atggtgttca atgttttgct agatacccag atcatatgaa
     1321 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     1381 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     1441 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
     1501 caaattggaa tacaactata actctcacaa tgtttacatc actgctgaca aacaaaagaa
     1561 tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg ttcaattagc
     1621 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     1681 ttacttatcc tatcaatctg ccttattcaa agatccaaac gaaaagagag accacatggt
     1741 cttgttagaa tttttgactg ctgctggtat taccgagggt atgaatgaat tgtacaaagg
     1801 taccagcctg aacagccccg tggacagcta caacgaaggc gtgaagatca agccacagga
     1861 aatcagccct cctcctacgg ccaacctgga ccgctccaat gacaaagtct atgagaatgt
     1921 aaccgggctg gtgaaagctg tcatagagat gtccagtaaa atacagccag ctccgccaga
     1981 ggagtacgtg cccatggtaa aggaggttgg cttggcgctg agaaccttgc tagcaacagt
     2041 ggatgagtcg ctgccagtgc ttcctgcaag cacccacaga gagattgaga tggcccagaa
     2101 actgctgaac tctgacctgg ctgagctcat taacaagatg aagctggccc agcagtacgt
     2161 catgaccagc ctgcagcagg agtacaagaa gcaaatgctg acggctgctc acgctctggc
     2221 tgtggatgcc aagaacttgc tggatgtcat cgatcaagcc agactgaaaa tgatcagcca
     2281 gtccaggccc cactaagaat tc
//
