LOCUS       sequence-149639-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, 
            Colecraft HM, Shaw RM
  TITLE     BIN1 localizes the L-type calcium channel to cardiac T-tubules.
  JOURNAL   PLoS Biol. 2010 Feb . 8(2):e1000312.
  PUBMED    20169111
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010 Feb . 8(2):e1000312."
COMMENT     Sequence Label: pDest-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(158..868)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(436..455)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     705..723
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      865..874
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    888..912
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      121 agagtcgcgg ccgctttact tgtacagctt cgaattctta cttgtacagc tcgtccatgc
      181 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      241 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      301 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      361 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      421 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      481 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
      541 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
      601 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
      661 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
      721 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
      781 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
      841 tgttatcctc ctcgcccttg ctcaccatgg tggcgggatc ccatcgaacc actttgtaca
      901 agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
//
