LOCUS       sequence-149641-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to subclone in gene of interest into blue motor neuron 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pTol2p2A--3mnx1:mTagBFP-NS-MCS
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     93..109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     93..109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    129..149
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtgtattagt cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg
       61 tgtctgaaac acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc
      121 caagctatca actttgtata gaaaagttgc tgaggaaaac gctttcgact acagaaggac
      181 tttgtattat gcaatgtttt caaaaaaata tcttcatatt cagtgtggga gataataaga
      241 aaggaaaaag catgaagcac tgaatcggtg tcctttaatg acttttatag ttgggaacaa
      301 ttagccgttc tccacaaatt aaattgataa aaagattacg agatccaaat gcgtttaaaa
      361 gctgtggtac gcacggccca tttgtgcaat tatgatcgaa aatatttatt gcaataaaag
      421 agacagtgca ggtcccgtta aattggcttt tcataatgaa cacatttaac atggataatt
      481 aatatataaa ctatttcgat ttgtcacgat gatatgtgtt gcacttaatt gtgaaagtaa
      541 ttaccgagcg aatgaacatc tttgaggaga gtccctggtt tcaggccctc gtatcatcgg
      601 gtgcaataat aattttactc tctcctgatg tgatgaaaaa gagccataaa tctctcagat
      661 cgctgcaggt ccacggcttc atatttcacc gctgctttaa tagaaatatt catgcgatcc
      721 cttaatgaaa ttaaaactga gaagcggaca aagcggccct aggctatgtg caacgtgtaa
      781 agacacaaga gcatttgatt gggaaaatgt tgcatccttc aaatatgagg atgttgttgc
      841 actgtaaacc acgtaaaaca attttttttc ttacagtcca gtattggcca cacttga
//
