LOCUS       sequence-149642-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to subclone in gene of interest into blue motor neuron 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pTol2p2A--3mnx1:mTagBFP-NS-MCS
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(11..35)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccatggtggc agcctgcttt tttgtacaaa cttgccacct cacaaacaga ttaacgcgtc
       61 gttcgtaact ttaaaaggct gtcaggagtg tcaccgctcc tcttcgtaaa gttatgatct
      121 tggccaaggt gtgcaagcca gaagtccact tgtcaactgc tgcccgagag ggagtggatt
      181 ggtcattcac gtcattcttg tgcatttccg attggacagt gattccagtg ttcacggtgt
      241 gcttgtagaa gttcaagagt tgtcataagt atgttctccc ataaaatgaa agctttttaa
      301 gtgggaaaca caacatgcac acatacattt tacatctact taatttatga ataattccag
      361 atttgtagag tctaaagggc aattattaac atgcaacttt gaaacatgaa acatttataa
      421 agttatcaag atttcattat ttaaacaccg acctataatc ttaatataaa cgaagattga
      481 taaagcacgt taactcgaaa atattatttt ggcgaaatag tttgtttgaa caaaaaaaaa
      541 aaacagccat gcattttatt cgcatagatg tagaggcatg acattttttt catttgtata
      601 attttttttt atttcatcaa attaattaac aaaaaaccat agtctataca cgcatttgtc
      661 attttgctat gacaccattt taatgtccat acttttttat tcaattgtat ttttttatct
      721 gaacagcctg cgataataaa aaaaactatt tgattttcac attgatgttg tacacgtata
      781 ttttttgttt ccccaaagaa ccttttagag gacagttctt aaaagaatta ctatctttat
      841 gagaagagaa ccattttaaa tgtcaaacgg atcttttaca ttacgatgaa cattttgcgc
      901 agaggaaata ttccagttat tccttatttg ttagagagca ttaggtaggc aaaatttaat
      961 aaccatgaac caat
//
