LOCUS       sequence-149643-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to subclone in gene of interest into blue motor neuron 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pTol2p2A--3mnx1:mTagBFP-NS-MCS
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..28
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    36..56
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(96..112)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(97..113)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(148..849)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     regulatory      846..855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(856..880)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcgatggtac cgtaaaacga cggccagtga attatcaact atgtataata aagttgcact
       61 agttctagac ccggggaatt cgatatcaag cttatcgata ccgtcgacct cgagggatcc
      121 agccactttg tacaagaaag ctgggtcatt aagcttgtgc cccagtttgc tagggaggtc
      181 gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt
      241 gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg
      301 tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga tggctcccgc ccacgagctt
      361 cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa
      421 ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc
      481 tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt
      541 cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga
      601 ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag
      661 gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat
      721 tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg
      781 atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc
      841 gctcaccatg gtggcagcct gcttttttgt acaaacttgc cacctcacaa acagattaac
      901 gcgtcgttcg taactttaaa aggctgtcag gagtgtcacc gctcctcttc gtaaagttat
      961 gatcttggcc aaggtgtgca agccagaagt ccacttgtca actgctgccc gagagggagt
     1021 ggattggtca ttcacgtcat tcttgtgcat ttccgattgg acagtga
//
