LOCUS       sequence-149646-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9, inducible expression of the Red 
            recombineering system, and inducible expression of the plasmid 
            curing system for CRISPR mediated genome editing of E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pREDCas9
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        468..546
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 tctctccact gtcaaataaa agagccccta taagattttt tttgatactg tggcggtctg
       61 tatttcccag aaccttgaac tttttagacg gaaccttata ttcatcagtg atcaccgccc
      121 atccgacgct atttgtgccg atatctaagc ctattgagta tttcttatcc atttttgcct
      181 cctaaaataa aaagtttaaa ttaaatccat aatgagtttg atgatttcaa taatagtttt
      241 aatgacctcc gaaattagtt taatatgctt taatttttct ttttcaaaat atctcttcaa
      301 aaaatattac ccaatactta ataataaata gattataaca caaaattctt ttaaaaagta
      361 gtttattttg ttatcattct atagtattaa gtattgtttt atggctgata aatttctttg
      421 aatttctcct tgattatttg ttataaaagt tataaaataa tcttgttgga accattcaaa
      481 acagcatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      541 cggtgctttt tttgatactt ctattctact ctgactgcaa accaaaaaaa caagcgcttt
      601 caaaacgctt gttttatcat ttttagggaa attaatctct taatcctttt atcattctac
      661 atttaggcgc tgccatcttg ctaaacctac taagctccac aggatgattt cgtaatcccg
      721 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcaagat ccgcatgcgg
      781 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
      841 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
      901 agcctgcgcg agcagcggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      961 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1021 tctacacgac ggggagtcag
//
