LOCUS       sequence-149647-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9, inducible expression of the Red 
            recombineering system, and inducible expression of the plasmid 
            curing system for CRISPR mediated genome editing of E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pREDCas9
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   198..233
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(234..365)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 gtgagggttt gcaactgcgg gtcaaggatc tggatttcga tcacggcacg atcatcgtgc
       61 gggagggcaa gggctccaag gatcgggcgc gaaaaaaccc cgccctgtca ggggcggggt
      121 tttttgcgga gcttagtaaa gccctcgcta gattttaatg cggatgttgc gattacttcg
      181 ccaactattg cgctgcagtt ttgggaccat tcaaaacagc atagctctaa aacctcgtag
      241 actatttttg tctaaaaaat ttcgtaatcg cactatttgt ctcagctaga cttcagtctt
      301 gaaaagcccc tgtattactg catttattaa gagtattata ccatattttt agttattaag
      361 aaataatctt catctaaaat atacttcagt cacctcctag ctgactcaaa tcaatgcgtg
      421 tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact tcttttgtag
      481 acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg ggagctccaa
      541 gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt ggtttgtctc
      601 tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa ataacacgct
      661 tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc tgctccacaa
      721 acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa tgactagcta
      781 aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt tgtaattctc
      841 cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta tatttaggta
      901 gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa aagtcaatcg
      961 gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct ttaacggatt
     1021 ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa taagctaccg
     1081 ttggactatc aaaaccacca tat
//
