LOCUS       sequence-149648-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c2-FL3
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      568..765
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(825..842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcttgacgac acggcttacc ggtaccgcac cagtgtcccg ggggacgccg aggccatcga
       61 ggcactggat gggtccttca ccaccgacac cgtcttccgc gtcaccgcca ccggggacgg
      121 cttcaccctg cgggaggtgc cggtggaccc gcccctgacc aaggtgttcc ccgacgacga
      181 atcggacgac gaatcggacg acggggagga cggcgacccg gactcccgga cgttcgtcgc
      241 gtacggggac gacggcgacc tggcgggctt cgtggtcgtc tcgtactccg gctggaaccg
      301 ccggctgacc gtcgaggaca tcgaggtcgc cccggagcac cgggggcacg gggtcgggcg
      361 cgcgttgatg gggctcgcga cggagttcgc ccgcgagcgg ggcgccgggc acctctggct
      421 ggaggtcacc aacgtcaacg caccggcgat ccacgcgtac cggcggatgg ggttcaccct
      481 ctgcggcctg gacaccgccc tgtacgacgg caccgcctcg gacggcgagc aggcgctcta
      541 catgagcatg ccctgcccct aatcagtact gacaataaaa agattcttgt tttcaagaac
      601 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
      661 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
      721 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
      781 taacgccgcc atccagtgtt aaacgagctc gaattctagg cttcctcgct cactgactcg
      841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      901 ttatccaca
//
