LOCUS       sequence-149650-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9, inducible expression of the Red 
            recombineering system, and inducible expression of the plasmid 
            curing system for CRISPR mediated genome editing of E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pREDCas9
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(415..1095)
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
ORIGIN
        1 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
       61 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
      121 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
      181 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
      241 ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa
      301 tcgcaacatc cgcattaaaa tctagcgagg gctttactaa gctcctgcag agatctgaat
      361 tccctagaga gacgaaagtg attgcgccta cccggatatt atcgtgagga tgcgtcatcg
      421 ccattgctcc ccaaatacaa aaccaatttc agccagtgcc tcgtccattt tttcgatgaa
      481 ctccggcacg atctcgtcaa aactcgccat gtacttttca tcccgctcaa tcacgacata
      541 atgcaggcct tcacgcttca tacgcgggtc atagttggca aagtaccagg cattttttcg
      601 cgtcacccac atgctgtact gcacctgggc catgtaagct gactttatgg cctcgaaacc
      661 accgagccgg aacttcatga aatcccggga ggtaaacggg catttcagtt caaggccgtt
      721 gccgtcactg cataaaccat cgggagagca ggcggtacgc atactttcgt cgcgatagat
      781 gatcggggat tcagtaacat tcacgccgga agtgaattca aacagggttc tggcgtcgtt
      841 ctcgtactgt tttccccagg ccagtgcttt agcgttaact tccggagcca caccggtgca
      901 aacctcagca agcagggtgt ggaagtagga cattttcatg tcaggccact tctttccgga
      961 gcggggtttt gctatcacgt tgtgaacttc tgaagcggtg atgacgccga gccgtaattt
     1021 gtgccacgca tcttccccct gttcgacagc tctcacatcg atcccggtac gctgcaggat
     1081 aatgtccggt gtcatgctgc caccttctgc tctgcggctt tctgtttcag gaatcca
//
