LOCUS       sequence-149654-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive Cre expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNXRVat-HACre
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             160..186
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             205..225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta ggatctcgag ctcaagcttc gtacgctgca
       61 ggtcgacgga tccccgggtt aattaacgct agcatgtacc catacgatgt tcctgactat
      121 gcgggctacc cgtatgacgt gccggactat gcaggagcct atccttatga cgttccagat
      181 tacgctggtt ctagatgtac tccaccaaag aagaagagaa aggttgcccc tagcatggcc
      241 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
      301 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
      361 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
      421 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
      481 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc
      541 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
      601 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
      661 gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca
      721 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
      781 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
      841 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
      901 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
      961 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
     1021 gggatttttg aagcaactca tcgattgatt tacgg
//
