LOCUS       sequence-149659-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to subclone in tagged gene of interest into inducible 
            motor neuron expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: 
            pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    267..401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(291..310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     345..364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(455..488)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(492..516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      518..527
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacgcca agaccacata
       61 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
      121 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
      181 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattaag gcgcgcctct
      241 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
      301 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      361 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      421 attttatgtt tcaggttcag ggggaggtgt gggaataact tcgtatagca tacattatac
      481 gaagttatac tacccagctt tcttgtacaa agtggctgcc accatggtgt ccaagggcga
      541 agaggacaac atggccatca tcaaagagtt catgcggttc aaggtgcaca tggaaggcag
      601 cgtgaacggc cacgagttcg agattgaggg cgagggcgaa ggcagaccct acgagggaac
      661 acagaccgcc aagctgaaag tgaccaaggg cggacccctg cccttcgcct gggatattct
      721 gagcccccag tttatgtacg gcagcaaggc ctacgtgaag caccccgccg acatccccga
      781 ctacctgaag ctgagcttcc ccgagggctt caagtgggag agagtgatga acttcgagga
      841 cggcggcgtc gtgaccgtga cccaggatag ctctctgcag gacggcgagt tcatctacaa
      901 agtgaagctg cggggcacca acttccccag cgacggaccc gtgatgcaga aaaagaccat
      961 gggctgggag gccagcagcg agagaatgta ccctgaggat ggcgccctga agggcgagat
     1021 caagcagcgg ctgaaactga aggatggcgg cc
//
