LOCUS       sequence-149660-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to subclone in tagged gene of interest into inducible 
            motor neuron expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: 
            pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..49
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(52..76)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcgctcatg gtggcataac ttcgtataat gtatgctata cgaagttatt aagcctgctt
       61 ttttgtacaa acttgccacc tcacaaacag attaacgcgt cgttcgtaac tttaaaaggc
      121 tgtcaggagt gtcaccgctc ctcttcgtaa agttatgatc ttggccaagg tgtgcaagcc
      181 agaagtccac ttgtcaactg ctgcccgaga gggagtggat tggtcattca cgtcattctt
      241 gtgcatttcc gattggacag tgattccagt gttcacggtg tgcttgtaga agttcaagag
      301 ttgtcataag tatgttctcc cataaaatga aagcttttta agtgggaaac acaacatgca
      361 cacatacatt ttacatctac ttaatttatg aataattcca gatttgtaga gtctaaaggg
      421 caattattaa catgcaactt tgaaacatga aacatttata aagttatcaa gatttcatta
      481 tttaaacacc gacctataat cttaatataa acgaagattg ataaagcacg ttaactcgaa
      541 aatattattt tggcgaaata gtttgtttga acaaaaaaaa aaaacagcca tgcattttat
      601 tcgcatagat gtagaggcat gacatttttt tcatttgtat aatttttttt tatttcatca
      661 aattaattaa caaaaaacca tagtctatac acgcatttgt cattttgcta tgacaccatt
      721 ttaatgtcca tactttttta ttcaattgta ttttttatct gaacagcctg cgataataaa
      781 aaaaactatt tgattttcac attgatgttg tacacgtata ttttttgttt ccccaaagaa
      841 ccttttagag gacagttctt aaaagaatta ctatctttat gagaagagaa ccattttaaa
      901 tgtcaaacgg atcttttaca tt
//
