LOCUS       sequence-149662-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GentR version of pRIM2 carrying rfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pRIM3-rfp
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(48..53,54..69,70..76)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    84..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     89..111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
       61 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagaat
      121 tgaaaggagg acaccatggc gagtagcgaa gacgttatca aagagttcat gcgtttcaaa
      181 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
      241 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
      301 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
      361 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
      421 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
      481 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
      541 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
      601 gctctgaaag g
//
