LOCUS       sequence-149673-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane targeted TagBFP (blue) fluorophore for use with 5' 
            promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-mTagBFP-CAAX
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             107..805
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(891..990)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc ttacccgggc gccaccatga gcgagctgat
      121 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
      181 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      241 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
      301 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
      361 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      421 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      481 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
      541 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
      601 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
      661 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
      721 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
      781 tagcaaactg gggcacaagc ttaatggagg aggaagatct aagctgaacc ctcctgatga
      841 gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgaactagtt acccagcttt
      901 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
      961 cactatcagt caaaataaaa tcattatttg ccatcc
//
