LOCUS       sequence-149688-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive Cre expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNXRVa-HACre
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..121
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             125..151
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             158..184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             203..223
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagg atctcgagct caagcttcgt acgctgcagg
       61 tcgacggatc cccgggttaa ttaacgctag catgtaccca tacgatgttc ctgactatgc
      121 gggctacccg tatgacgtgc cggactatgc aggagcctat ccttatgacg ttccagatta
      181 cgctggttct agatgtactc caccaaagaa gaagagaaag gttgccccta gcatggccaa
      241 tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga gtgatgaggt
      301 tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc atacctggaa
      361 aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata accggaaatg
      421 gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg cgcgcggtct
      481 ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtccgg
      541 gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga
      601 aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga
      661 ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta atctggcatt
      721 tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa
      781 agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct
      841 ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg
      901 atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag
      961 aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg
     1021 gatttttgaa gcaactcatc gattgattta cggc
//
