LOCUS       sequence-149689-        4445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Degradation tagged CFP fluorescent reporter for MarA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   El_Meouche I, Siu Y, Dunlop MJ
  TITLE     Stochastic expression of a multiple antibiotic resistance activator 
            confers transient resistance in single cells.
  JOURNAL   Sci Rep. 2016 Jan 13;6:19538. doi: 10.1038/srep19538.
  PUBMED    26758525
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-01-13"; volume: "6:19538. doi"; pages: " 
            10.1038/srep19538"
COMMENT     Sequence Label: PmarA-cfp
FEATURES             Location/Qualifiers
     source          1..4445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             162..173
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      985..1056
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1072..1099
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1630..2580)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3342..3436)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3467..4261)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3578..3597)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4188..4207
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgcatcgcat tgaacaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt
       61 catttgactt atacttgcct gttacctatt atcccctgca actaattacg gtaaagggca
      121 actaatgtga aaagtaccag cgatctggga tccttaactt taaagaggag aaaggtaccg
      181 catgactagc aaaagaagca aaggtgaaga actgttcact ggtgttgttc caattctggt
      241 tgaactggat ggtgatgtta atggtcacaa attttctgtc tctggtgagg gtgaaggtga
      301 tgcaacctac ggtaaactga ccctgaaatt tatttgcact actggtaaac tgcctgttcc
      361 gtggccaacc ctggtcacta ctctgacttg gggtgttcaa tgctttgctc gttacccaga
      421 tcacatgaaa cagcatgact ttttcaagtc tgccatgccg gaaggttatg ttcaggaacg
      481 tactatcttt ttcaaagatg acggtaacta caagacccgt gctgaagtca agtttgaagg
      541 tgataccctg gttaatcgta tcgagctgaa aggtattgat tttaaagaag atggtaacat
      601 tctgggtcac aaactggaat acaacgctat ttctgataat gtatacatca ctgctgacaa
      661 acaaaagaat ggtatcaaag ctaatttcaa aattcgtcac aacattgaag atggtagcgt
      721 tcaactggca gaccattatc aacaaaatac tccaattggc gatggccctg tcctgctgcc
      781 agacaaccat tacctgtcca cccaatctcg tctgtctaaa gatccgaacg aaaagcgcga
      841 tcacatggtc ctgctggagt ttgtaaccgc tgctggtatt accctgggca tggatgaact
      901 gtataaaact agtgcagcga acgacgaaaa ttacgccctt gcagcgtaat agggatccaa
      961 actcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1021 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1081 tcgggtgggc ctttctgcgt ttatacctag ggtacgggtt ttgctgcccg caaacgggct
     1141 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc
     1201 tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     1261 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     1321 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     1381 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     1441 atctgttcat ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct
     1501 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg
     1561 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     1621 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     1681 ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa
     1741 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     1801 tagtccgtta tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     1861 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     1921 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     1981 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     2041 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     2101 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     2161 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     2221 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     2281 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     2341 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     2401 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     2461 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     2521 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     2581 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     2641 ataattacta gtccttttcc cgggtgatct gggtatctgt aaattctgct agacctttgc
     2701 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     2761 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     2821 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     2881 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     2941 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     3001 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     3061 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     3121 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     3181 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     3241 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     3301 tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac
     3361 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     3421 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt
     3481 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     3541 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     3601 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     3661 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     3721 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     3781 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     3841 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     3901 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     3961 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     4021 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     4081 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     4141 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     4201 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     4261 tgcgaaacga tcctcatcct gtctcttgat cagatcatga tcccctgcgc catcagatcc
     4321 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     4381 ccccagctgg caattccgac gtcggtgcct aatgagtgag ctaacttaca ttaattgcgt
     4441 tgcgc
//
