LOCUS       sequence-149691-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive Cre expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNXRVa-HACre
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..805
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="KEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDA
                     PELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQ
                     VLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFD
                     DERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRY
                     QDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(63..82)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata
       61 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
      121 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      181 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      241 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa
      301 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      361 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
      421 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
      481 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      541 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
      601 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
      661 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
      721 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
      781 tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag
      841 aacttgtcat ttgtatagtt tttttatatt g
//
