LOCUS       sequence-149699-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized LshC2c2 for purification in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
            DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, 
            Regev A, Lander ES, Koonin EV, Zhang F
  TITLE     C2c2 is a single-component programmable RNA-guided RNA-targeting 
            CRISPR effector.
  JOURNAL   Science. 2016 Jun 2. pii: aaf5573.
  PUBMED    27256883
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 2. pii: aaf5573."
COMMENT     Sequence Label: pC001 - huLshC2C2-MBP for bacterial expression
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..64)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(88..168)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(175..222)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gccattcaat tttctgggct tcaaagatat cgttcaggcc tttatcatca tcatctttat
       61 aatcgctacc accaccctcg aggcttaggc atagtcgggg acatcatatg ggtatgcata
      121 atcaggcacg tcgtagggat aagcgtaatc tggaacatcg tatgggtagg atcccttttt
      181 cttttttgcc tggccggcct ttttcgtggc cgccggcctt ttcagggtat cgttagtatt
      241 ctcgattttg gtcagcagtt caatgatcag attcttaatg tagtcactgt tgtaggactc
      301 cagttccagg acgctcactt tcttgggctt catcaggcgc tccagaatat cattgttgcc
      361 gatcagtttg aatttctttt tcagctcatc gtagtccaga ttcacgtcct ttttaaagac
      421 ttcgaacacg ctggcataag ttgaattgtt gtagcgtgtg ctatatgaca gcaggttgct
      481 cacccgatcg atctgctcag caatactgta gtcggcaaat ggatttctca cgatgtagaa
      541 atgggagata tagttccgaa tgctctcatt ttcaggcttg ttgatctcgg aattttcgct
      601 caggtcgatt ccaaacccgt agcaaatctt ctcgaacttc ttgtaggact cttcatcaaa
      661 gaacttgta
//
