LOCUS       sequence-149701-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a 
            sgRNA driven by a U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Nymark M, Sharma AK, Sparstad T, Bones AM, Winge P
  TITLE     A CRISPR/Cas9 system adapted for gene editing in marine algae.
  JOURNAL   Sci Rep. 2016 Apr 25;6:24951. doi: 10.1038/srep24951.
  PUBMED    27108533
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-25"; volume: "6:24951. doi"; pages: " 
            10.1038/srep24951"
COMMENT     Sequence Label: pKS diaCas9_sgRNA
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(565..612)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttaacttg ctatttctag ctctaaaact gagaccctct ctcggtctcc tcgactttga
       61 aggtgttttt tgaccttata aagctctaaa atattgttga ctaacaatga atacttggcg
      121 tgtcaaagta ccttgtagat attatagatc ctattcaatt taccgtcaat tcgacattaa
      181 aagcgctaag gattgaaaat gagcatattg acagtgaggc tgtcgagaac gaaccgagtt
      241 tcacttcctt ctgtcctccg ttttcttctc gctagcagtg accgacaacc aatttccagc
      301 tcaccgtcaa caccaacttc cgagccaacc tgcagtgaag acgagctagt gttattcctg
      361 actgtgaaac caaagcggag tgactgcaac taatgaaaat taaattttta aggaaggata
      421 gagactccca actgttcgtg caccatgaga gcaaggatgc ccatttgtgc cgcacgtccc
      481 tggttgagtt cgatagcacg cttctggagc ttcttgtcct cactgaaagt gtcccagcca
      541 aagtcgaggt agggatccag attacttttt cttttttgcc tggccggcct ttttcgtggc
      601 cgccggcctt ttgtcgcctc cgagttgaga caggtcgatg cgtgtctcgt acaagccggt
      661 gattgattgg tgaatgaggg tggcgtccaa gacttctttg gtgctggtgt acctcttgcg
      721 gtcgatggtg gtgtcaaagt acttaaaggc ggcaggggct ccaagattgg tgagcgtaaa
      781 caagtggata atattctccg cttgctctcg gattggctta tcgcggtgct tattgtaggc
      841 ggacaggact ttgtccagat tagcgtcggc aaggatgact cgcttggaga actcgctaat
      901 ttgctca
//
