LOCUS       sequence-149704-       12080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacillus subtilis dCas9 expression vector; integrates into 
            lacA/ganA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     Sequence Label: pJMP1
FEATURES             Location/Qualifiers
     source          1..12080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1087..1278
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1272..1294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1380..1520
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1435..1454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1706..2294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1786..1805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2465..3256,3257..3325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3088..3107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3326..3430)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      4074..4108
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             join(5580..5606,5607..5609,5610..8096,8097..8099,
                     8100..9686)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      9710..9781
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9797..9824
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(9899..10003)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      11289..11375
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11394..11421
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      11501..11535
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 atcctgatat tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg
       61 aattagtaga aaagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg
      121 gatttgacgg cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc
      181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
      241 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
      301 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
      361 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
      421 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
      481 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
      541 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
      601 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
      661 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
      721 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
      781 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
      841 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
      901 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
      961 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     1021 cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg
     1081 catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     1141 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     1201 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     1261 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1321 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1381 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     1441 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1501 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2761 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3061 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     3121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaatgatggg
     3541 ctaatgtgtg tttacgacaa ttctcacttc atacttttcc atcgtcaggt cgcctgacaa
     3601 tatgtctcct gtcattatgt ccttcacact ctgatcaaac gtgaccagct gtttttcttc
     3661 cgtgaaattc atgacaaaaa tataatcatt gtcctgatcc tgcctcgctt gtacggagac
     3721 gccttttccg tgccgaaccg gaaaaactgg agagagagac aggtctgtga tcagaccctc
     3781 atagaaatca cgctgaaatt gatcctccaa acgcgcgccg ataaaatacg ccttgccctg
     3841 ctgatactca tggcttgtga ccgctggcgt gcgcgcataa aaatcttctt gatacaccgc
     3901 ttccactgaa gctgtcttta catcaatcac ggttgcataa tccttcattt catatatttg
     3961 gctgcggtag ctgacagcgt ttcgatcctt cggatacagg gtgtccgttt caagaggctc
     4021 aactccaaat atagcttgaa atcgatatct ctgcagtcgc gatgattaat taattcagaa
     4081 cgctcggttg ccgccgggcg ttttttatgc agcaatggca agaacgtccc ggggagctcc
     4141 taacttatag gggtaacact taaaaaagaa tcaataacga tagaaaccgc tcctaaagca
     4201 ggtgcatttt ttcctaacga agaaggcaat agttcacatt tattgtctaa atgagaatgg
     4261 actctagaag aaacttcgtt tttaatcgta tttaaaacaa tgggatgaga ttcaattata
     4321 tgatttctca agataacagc ttctatatca aatgtattaa ggatattggt taatccaatt
     4381 ccgatataaa agccaaagtt ttgaagtgca tttaacattt ctacatcatt tttatttgcg
     4441 cgttccacaa tctcttttcg agaaatattc ttttcttctt tagagagcga agccagtaac
     4501 gctttttcag aagcatataa ttcccaacag cctcgatttc cacagctgca tttgggtcca
     4561 ttaaaatcta tcgtcatatg acccatttcc ccagaaaaac cctgaacacc tttatacaat
     4621 tcgttgttaa taacaagtcc agttccaatt ccgatattaa tactgatgta aacgatgttt
     4681 tcatagtttt ttgtcatacc aaatactttt tcaccgtatg ctcctgcatt agcttcattt
     4741 tcaacaaaaa ccggaacatt aaactcactc tcaattaaaa actgcaaatc tttgatattc
     4801 caatttaagt taggcatgaa aataatttgc tgatgacgat ctacaaggcc tggaacacaa
     4861 attcctattc cgactagacc ataaggggac tcaggcatat gggttacaaa accatgaata
     4921 agtgcaaata aaatctcttt tacttcacta gcggaagaac tagacaagtc agaagtcttc
     4981 tcgagaataa tatttccttc taagtcggtt agaattccgt taagatagtc gactcctata
     5041 tcaataccaa tcgagtagcc tgcattctta ttaaaaacaa gcattacagg tcttctgccg
     5101 cctctagatt gccctgcccc aatttcaaaa ataaaatctt tttcaagcag tgtatttact
     5161 tgagaggaga cagtagactt gtttaatcct gtaatctcag agagagttgc cctggagaca
     5221 ggggagttct tcaaaatttc atctaatatt aatttttgat tcattttttt tactaaagct
     5281 tgatctgcaa tttgaataat aaccactcct ttgtttatcc accgaactaa gttggtgttt
     5341 tttgaagctt gaattagata tttaaaagta tcatatctaa tattataact aaattttcta
     5401 aaaaaaacat tgaaataaac atttattttg tatatgatga gataaagtta gtttattgga
     5461 taaacaaact aactcaatta agatagttga tggataaact tgttcactta aatcaaaggg
     5521 ggaaatgaca aatggtccaa actagtgata tctaaaaatc aaagggggaa atgggatcca
     5581 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
     5641 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
     5701 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
     5761 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
     5821 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
     5881 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
     5941 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
     6001 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
     6061 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
     6121 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
     6181 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
     6241 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
     6301 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
     6361 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
     6421 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
     6481 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
     6541 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
     6601 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
     6661 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
     6721 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
     6781 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
     6841 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
     6901 aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc
     6961 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
     7021 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
     7081 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
     7141 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
     7201 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
     7261 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
     7321 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     7381 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     7441 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     7501 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
     7561 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     7621 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     7681 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     7741 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     7801 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     7861 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
     7921 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     7981 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     8041 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca
     8101 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
     8161 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     8221 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     8281 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     8341 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     8401 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     8461 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     8521 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
     8581 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     8641 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
     8701 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     8761 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     8821 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
     8881 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
     8941 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
     9001 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
     9061 aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact
     9121 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
     9181 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
     9241 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
     9301 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
     9361 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
     9421 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
     9481 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
     9541 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
     9601 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
     9661 atttgagtca gctaggaggt gactaactcg agtaaggatc tccaggcatc aaataaaacg
     9721 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     9781 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata cctagggata
     9841 tattccgctt cctcgctcac tgactcggtc tcggatccga gctcccgcgg gcggccgcac
     9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10081 tcacgaggcc ctttcgtctt caagaattga tcctctagca caaaagaaaa acgaaatgat
    10141 acaccaatca gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac
    10201 ttgcaccata tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg
    10261 gaaataagac ttagaagcaa acttaagagt gtgttgatag tgcagtatct taaaattttg
    10321 tataatagga attgaagtta aattagatgc taaaaatttg taattaagaa ggagtgatta
    10381 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
    10441 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
    10501 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
    10561 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
    10621 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggag
    10681 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
    10741 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
    10801 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
    10861 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
    10921 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
    10981 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
    11041 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
    11101 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
    11161 ctaaagggaa tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga
    11221 ggtccctaga ctctagaccc ggggatctct gcagtgagat ctggtaatga ctctctagct
    11281 tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
    11341 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagcta agcagaaggc
    11401 catcctgacg gatggccttt ttgcgtttct acaaactctt gttaactcta gagctgcctg
    11461 ccgcgtttcg gtgatgaaga tcttcccgat gattaattaa ttcagaacgc tcggttgccg
    11521 ccgggcgttt tttatgcagc aatggcaaga acgttgctct agagcggccg catcgattca
    11581 cagtggcaat ctcccccgta ttcgtttgaa atgtgccaca ttaacagcgc cgggtgatgt
    11641 ccgtatcgtt ctgctaataa gcggttgatg tgccgtgttt tttctcggta gactttagat
    11701 gtgaggcagt ggttgtgcct tccgccgtgc agctgtttga cgcgggaggc attgacgcgc
    11761 aaaacttccg gataggtttg cgacagccag gccggacggg ctccgctcgg cgttgctaat
    11821 atgacccggc cgcctatact gtgaatccgc tcaaaaatat catccagcca ttcaaattga
    11881 tatacgccct cctccggctc aagtgcgctc catgcaaaaa tgccgacaga aaacgtattc
    11941 gtatgagaaa gcttcatcag tttgatatcg tcagctaaaa tatcgggccg atccagccac
    12001 tgatcggggt tgtagtctcc cccatggagc ataaattttg cttttgttac gtgcgttttt
    12061 tcaagctttg acatcacccc
//
