LOCUS       sequence-149725-         292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human OXSR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: OXSR1 gRNA (BRDN0001148375)
FEATURES             Location/Qualifiers
     source          1..292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        154..229
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaagtaataa ctttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
       61 atatgcttac cgctaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      121 ggacgaaaca ccgaattgaa ttggctacag ggggttttag agctagaaat agcaagttaa
      181 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
      241 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aa
//
