LOCUS       sequence-149726-        4701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of 
            yeast genes with yeast-adapted mTagRFP-T and G418 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM572
FEATURES             Location/Qualifiers
     source          1..4701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            73..1429
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        73..416
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             417..1226
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(484..503)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1232..1429
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1533..1552)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1534..1552)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1639..1656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1810..2398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1890..1909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2569..3360,3361..3429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3192..3211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3430..3534)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3602..3620
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3658..3680)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3780..3799
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3880..3898
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3880..3897
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt
       61 cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc
      121 tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca
      181 tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg
      241 cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg
      301 cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt
      361 ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg
      421 gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat gctgatttat
      481 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
      541 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      601 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
      661 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggca
      721 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      781 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
      841 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
      901 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      961 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     1021 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1081 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     1141 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     1201 atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa
     1261 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     1321 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     1381 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     1441 atactaacgc cgccatccag tttaaacgag ctcgaattca tcgatgatat cagatccact
     1501 agtggcctat gcggccgcgg atctgccggt ctccctatag tgagtcgtat taatttcgat
     1561 aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1621 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1681 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1741 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1801 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1861 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1921 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1981 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     2041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2221 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     2281 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2341 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2401 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2461 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2521 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2581 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2641 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2701 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2761 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2821 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2881 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2941 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3001 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3061 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3121 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3181 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3241 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3301 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3361 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3421 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3481 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3541 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3601 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     3661 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     3721 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     3781 ggcatcagag cagattgtac tgagagtgca ccatatggac atattgtcgt tagaacgcgg
     3841 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaac
     3901 gcggccgcca gctgaagctt cgtacgctgc aggtcgacgg tgcaggtgct ggagctggtg
     3961 ctggtatggt atctaagggt gaagaattga ttaaggaaaa catgcacatg aagttgtata
     4021 tggaaggtac tgtaaataac caccatttta agtgtacctc tgaaggtgaa ggtaaaccat
     4081 atgaaggtac acaaaccatg agaattaaag ttgtagaagg tggtccattg ccttttgcct
     4141 tcgatatctt agctacatct tttatgtacg gttcaagaac cttcatcaat catactcaag
     4201 gtataccaga tttctttaag caatcattcc ctgaaggttt cacttgggaa agagtcacta
     4261 catatgaaga cggtggtgtt ttgactgcca cacaagatac atccttgcaa gacggttgtt
     4321 taatctataa cgttaagatc agaggtgtaa acttcccaag taacggtcct gtaatgcaaa
     4381 agaaaacttt gggttgggaa gcaaacaccg aaatgttgta tcctgccgat ggtggtttag
     4441 aaggtcgaac tgacatggca ttgaaattag ttggtggtgg tcacttaatt tgcaatttca
     4501 agaccactta cagatctaaa aagccagcta aaaacttgaa gatgcctggt gtatactacg
     4561 tcgatcatag attggaaaga attaaagaag cagacaagga aacatatgtt gaacaacacg
     4621 aagtcgctgt tgcaagatac tgcgatttgc cttccaagtt aggtcataag ttgaacggta
     4681 tggacgaatt gtataagtaa g
//
