LOCUS       sequence-149743-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad range bacterial fluorescence labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Barbier M, Damron FH
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
  JOURNAL   PLoS One. 2016 Mar 3;11(3):e0146827. doi: 
            10.1371/journal.pone.0146827. eCollection 2016.
  PUBMED    26937640
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-3"; volume: "11(3):e0146827. doi"; pages: " 
            10.1371/journal.pone.0146827. eCollection 2016"
COMMENT     Sequence Label: pUCP20T-ecfp
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(29..123)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(191..212)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     540..559
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caggtcgact ctagaggatc cagcttggat tctcaccaat aaaaaacgcc cggcggcaac
       61 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      121 gtccaagctc agctaattaa gcttattact tgtacagctc gtccatgccg agagtgatcc
      181 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      241 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      301 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      361 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
      421 ggctgatgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      481 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      541 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      601 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
      661 cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      721 agatgaactt cagggtcagc ttgccgtagg tgg
//
