LOCUS       sequence-149745-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad range bacterial fluorescence labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Barbier M, Damron FH
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
  JOURNAL   PLoS One. 2016 Mar 3;11(3):e0146827. doi: 
            10.1371/journal.pone.0146827. eCollection 2016.
  PUBMED    26937640
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-3"; volume: "11(3):e0146827. doi"; pages: " 
            10.1371/journal.pone.0146827. eCollection 2016"
COMMENT     Sequence Label: pUCP20T-tsapphire
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..743)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 gcctgcaggt cgactctaga ggatccttat ttgtacaatt catcaatacc atgggtaata
       61 ccagcagcag taacaaattc taacaagacc atgtggtctc tcttttcgtt tggatctttg
      121 gataaggcag attgaatgga taagtaatgg ttgtctggta acaagactgg accatcacca
      181 attggagtat tttgttgata atggtcagct aattgaacac caccatcttc aatgttgtgt
      241 ctaattttga agttagcttt gataccattc ttttgtttgt cagccatgat gtaaacattg
      301 tgagagttaa agttgtattc caatttgtga cctaaaatgt taccatcttc tttaaaatca
      361 atacctttta attcgattct attaactaag gtatcacctt caaacttgac ttcagctctg
      421 gtcttgtagt taccgtcatc tttgaaaaaa atagttcttt cttgaacata accttctggc
      481 atggcagact tgaaaaagtc atgttgtttc atatgatctg ggtatctagc aaaaaccata
      541 acaccataag aaaaagtagt gactaaggtt ggccatggaa ctggcaattt accagtagta
      601 caaataaatt ttaaggtcaa tttaccgtaa gtagcatcac cttcaccttc accggagaca
      661 gaaaatttgt gaccattaac atcaccatct aattcaacca aaattgggac aacaccagtg
      721 aataattctt cacctttaga catccttctt
//
