LOCUS       sequence-149780-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad range bacterial fluorescence labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Barbier M, Damron FH
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
  JOURNAL   PLoS One. 2016 Mar 3;11(3):e0146827. doi: 
            10.1371/journal.pone.0146827. eCollection 2016.
  PUBMED    26937640
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-3"; volume: "11(3):e0146827. doi"; pages: " 
            10.1371/journal.pone.0146827. eCollection 2016"
COMMENT     Sequence Label: pUCP20T-mPlum
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..712)
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(94..117)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(292..311)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgcatgcctg caggtcgact ctagaggatc cttaggcgcc ggtggagtgg cggccctcgg
       61 cgcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttga
      121 tgtcggtctt gtaggcgccg ggcagctgca cgggcttctt ggccatgtag gtggtcttga
      181 cctcggcgtc gtagtggccg ccgtccttca gcctcagcct catcttcatc tcgcccttca
      241 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
      301 gcattacggg gccgtcggag gggaagttgg tgccgcgcac cttcaccttg tagatgaact
      361 cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
      421 tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg
      481 cggggtgctt cacgtaggcc ttggagccgt acatgatctg aggggacagg atgtcccagg
      541 cgaagggcag ggggccaccc ttggtcacct tcagcctggc ggtctgggtg ccctcatagg
      601 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
      661 gctccttgaa gcgcatgaac tccttgatga cctcctcgcc cttgctcacc atccttcttg
      721 gcgcgc
//
