LOCUS       sequence-149786-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad range bacterial fluorescence labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Barbier M, Damron FH
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
  JOURNAL   PLoS One. 2016 Mar 3;11(3):e0146827. doi: 
            10.1371/journal.pone.0146827. eCollection 2016.
  PUBMED    26937640
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-3"; volume: "11(3):e0146827. doi"; pages: " 
            10.1371/journal.pone.0146827. eCollection 2016"
COMMENT     Sequence Label: pUCP30T-mKo1
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..683)
                     /codon_start=1
                     /product="Kusabira-Orange fluorescent protein (Karasawa et 
                     al., 2004)"
                     /label=Kusabira-Orange
                     /translation="VSVIKPEMKMKYFMDGSVNGHEFTVEGEGTGKPYEGHQEMTLRVT
                     MAKGGPMPFSFDLVSHTFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLQFEDGGFAA
                     VSAHISLRGNCFEHKSKFVGVNFPADGPVMQNQSSDWEPSTEKITTCDGVLKGDVTMYL
                     KLAGGGNHKCQFKTTYKAAKKILKMPQSHFIGHRLVRKTEGNITELVEDAVAHC"
     primer_bind     complement(715..731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(728..750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    739..755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(763..769,770..787,788..793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 catgcctgca ggtcgactct agaggatcct cagcaatgag ctactgcatc ttctaccagc
       61 tcagtaatgt tgccttcggt tttcctgacg aggcgatgcc cgatgaaatg gctttgtggc
      121 attttaagaa tcttttttgc cgccttgtaa gtagtcttga attggcattt gtgattgccg
      181 cctcccgcaa gttttaggta catcgtaaca tcacccttca gaactccgtc gcaggtagta
      241 attttctcgg ttgatggctc ccaatcagaa ctttggtttt gcatcacagg accatcggca
      301 ggaaagttaa ccccaacaaa tttggatttg tgctcgaagc agtttcctct aaggcttata
      361 tgcgcactga cggcagcgaa cccaccatct tcgaactgca acgacctttc ccatgacagg
      421 ccttcaggaa atgcttgttt gaaatagtct ggtatctctt ctggatattt agtaaaaggt
      481 ctgtggccgt aacagaacgt gtgtgacact aagtcaaacg agaaaggcat tggcccgccc
      541 ttggccattg tgacgcgtag tgtcatctct tgatgtccct cgtaaggttt gcctgtgcct
      601 tcaccttcaa ctgtgaactc atgcccattg acggagccgt ccatgaagta cttcatcttc
      661 atctctggtt taatcacact cacctccttc ttggcgcgcc gaattcgtaa tcatgtcata
      721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      781 cataaagtgt aaagcctgg
//
