LOCUS       sequence-149787-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human CCL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: CCL2 gRNA (BRDN0001147233)
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        207..282
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    338..455
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccgcagc caccttcatt ccccaagttt tagagctaga aatagcaagt taaaataagg
      241 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttaa gcttggcgta
      301 actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      361 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      421 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      481 atccactttg gcgccggctc gagggggccc gggtgcaaag atggataaag ttttaaacag
      541 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
      601 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggaggg
//
