LOCUS       sequence-149790-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane targeted KOFP2 (orange) fluorophore for use with 5' 
            promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-mKOFP2-CAAX
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctgaattcgc cgccaccatg gtgagtgtga
      121 ttaaacctga gatgaagatg cgctactaca tggacggatc tgtgaacggc cacgagttta
      181 ccatcgaggg cgagggaacc ggcagacctt acgagggaca ccaggaaatg accctgagag
      241 tcaccatggc cgagggcgga cctatgcctt tcgccttcga cctggtgtct cacgtgttct
      301 gctacggaca cagagtgttc accaagtacc ctgaggaaat ccccgactac ttcaagcagg
      361 ccttccctga gggactgagc tgggagagaa gcctggaatt tgaggacggc ggaagcgcta
      421 gcgtgtccgc ccacatcagc ctgagaggaa acaccttcta ccacaagagc aagttcacag
      481 gcgtgaactt ccctgccgac ggacctatca tgcagaacca gagcgtggac tgggagccta
      541 gcaccgagaa gatcaccgcc agcgacggcg tgctgaaggg cgacgtgacc atgtacctga
      601 agctggaagg cggaggcaac cacaagtgcc agatgaagac cacctacaag gccgccaaag
      661 aaatcctgga aatgccaggc gaccactaca tcggacacag actggtccgc aagaccgagg
      721 gaaacatc
//
