LOCUS       sequence-149798-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TagBFP (blue) fluorophore for use with 5' promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-mTagBFP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(120..821)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(837..861)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtaactagtt
      121 taattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac tgccacctcg
      181 tgctgctcga cgtaggtctc gttgttggcc tccttgattc tttccagtct gtagtccaca
      241 tagtagacgc caggcatctt gaggttctta gcgggtttct tggatctata tgtggtcttg
      301 atgtttgcga tcagatggct cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc
      361 aggccgccgt cagcggggta cagcgtctcg gtgaaggcct cccagccgag tgttttcttc
      421 tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt gtagatgagg
      481 cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg
      541 gtgactctct cccatgtgaa gccctcaggg aaggactgct tgaagaagtc ggggatgccc
      601 tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc tagtagccag gatgtcgaag
      661 gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt gccctcgtag
      721 ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt tgtccacggt gccctccatg
      781 tacagcttca tgtgcatgtt ctccttaatc agctcgctca tggtggcgcc cgggtaagcc
      841 tgcttttttg tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga
      901 acaggtcact at
//
