LOCUS       sequence-149800-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses maltose binding protein (MBP) SpyCatcher fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Min D, Arbing MA, Jefferson RE, Bowie JU
  TITLE     A simple DNA handle attachment method for single molecule mechanical
            manipulation experiments.
  JOURNAL   Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 
            2016 Jun 6.
  PUBMED    27222403
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2952"; 
            journalName: "Protein Sci"; date: "2016-08"; volume: "25"; issue: 
            "8"; pages: "1535-44"
COMMENT     Sequence Label: pMBP-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actttaagaa ggagatatac atatgaaaat cgaagaaggt aaactggtaa tctggattaa
       61 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
      121 aattaaagtc accgttgagc atccggataa actggaagag aaattcccac aggttgcggc
      181 aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca
      241 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
      301 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
      361 gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat
      421 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
      481 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
      541 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
      601 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
      661 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
      721 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
      781 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
      841 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
      901 ggttaataaa gacaaaccgc t
//
