LOCUS       sequence-149806-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 S199A  in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK3 S199A
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccgcc accatggatt acaaggatga cgacgataag
      121 agcgcgggcg gatccgaatt ctgcagatat ccatcacact ggcggccgct cgagatgtcg
      181 tgcgtcaagt tatggcccag cggtgccccc gcccccttgg tgtccatcga ggaactggag
      241 aaccaggagc tcgtcggcaa aggcgggttc ggcacagtgt tccgggcgca acataggaag
      301 tggggctacg atgtggcggt caagatcgta aactcgaagg cgatatccag ggaggtcaag
      361 gccatggcaa gtctggataa cgaattcgtg ctgcgcctag aaggggttat cgagaaggtg
      421 aactgggacc aagatcccaa gccggctctg gtgactaaat tcatggagaa cggctccttg
      481 tcggggctgc tgcagtccca gtgccctcgg ccctggccgc tcctttgccg cctgctgaaa
      541 gaagtggtgc ttgggatgtt ttacctgcac gaccagaacc cggtgctcct gcaccgggac
      601 ctcaagccat ccaacgtcct gctggaccca gagctgcacg tcaagctggc agattttggc
      661 ctgtccacat ttcagggagg ctcacagtca gggacagggt ccggggagcc agggggcacc
      721 ctgggctact tggccccaga actgtttgtt aacgtaaacc ggaaggccgc cacagccagt
      781 gacgtctaca gcttcgggat cctaatgtgg gcagtgcttg
//
