LOCUS       sequence-149816-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 2KR(89,501) in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK3 2KR(89,501)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcgagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttgccgccac catggattac aaggatgacg acgataagag
      121 cgcgggcgga tccgaattct gcagatatcc atcacactgg cggccgctcg agatgtcgtg
      181 cgtcaagtta tggcccagcg gtgcccccgc ccccttggtg tccatcgagg aactggagaa
      241 ccaggagctc gtcggcaaag gcgggttcgg cacagtgttc cgggcgcaac ataggaagtg
      301 gggctacgat gtggcggtca agatcgtaaa ctcgaaggcg atatccaggg aggtcaaggc
      361 catggcaagt ctggataacg aattcgtgct gcgcctagaa ggggttatcg agaaggtgaa
      421 ctgggaccaa gatccccggc cggctctggt gactaaattc atggagaacg gctccttgtc
      481 ggggctgctg cagtcccagt gccctcggcc ctggccgctc ctttgccgcc tgctgaaaga
      541 agtggtgctt gggatgtttt acctgcacga ccagaacccg gtgctcctgc accgggacct
      601 caagccatcc aacgtcctgc tggacccaga gctgcacgtc aagctggcag attttggcct
      661 gtccacattt cagggaggct cacagtcagg gacagggtcc ggggagccag ggggcaccct
      721 gggctacttg gccccagaac tgtttgttaa cgtaaaccgg aaggcctcca cagccagtga
      781 cgtctacagc ttcgggatcc taatgtgggc agtgcttgct
//
