LOCUS       sequence-149822-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP-KIF1B beta-600-1400.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Chen ZX, Wallis K, Fell SM, Sobrado VR, Hemmer MC, Ramskold D, 
            Hellman U, Sandberg R, Kenchappa RS, Martinson T, Johnsen JI, Kogner
            P, Schlisio S
  TITLE     RNA helicase A is a downstream mediator of KIF1Bbeta 
            tumor-suppressor function in neuroblastoma.
  JOURNAL   Cancer Discov. 2014 Apr;4(4):434-51. doi: 
            10.1158/2159-8290.CD-13-0362. Epub 2014 Jan 27.
  PUBMED    24469107
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0362"; journalName: "Cancer Discov"; date: 
            "2014-04"; volume: "4"; issue: "4"; pages: "434-51"
COMMENT     Sequence Label: GFP-KIF1B beta-600-1400
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(89..91,92..94,95..805)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(89..91,92..94,95..805)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(134..155)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(395..414)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     742..763
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcgagctct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 tactataggg agacccaagc ttggtaccat ggtgagcaag ggcgaggagc tgttcaccgg
      121 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      181 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      241 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      301 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      361 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      421 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      481 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      541 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      601 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      661 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      721 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      781 tctcggcatg gacgagctgt acaagggtac catgg
//
