LOCUS       sequence-149823-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c2-HA3
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..73
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      169..356
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(224..248)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     enhancer        381..684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        685..888
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     839..859
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     933..952
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        933..951
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcatgtacc catacgatgt
       61 tcctgactat gcgggctacc cgtatgacgt gccggactat gcaggagcct atccttatga
      121 cgttccagat tacgctggtt ctagatgagg cgcgccactt ctaaataagc gaatttctta
      181 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
      241 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
      301 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
      361 tccgctagga gatctcgcga cgttacataa cttacggtaa atggcccgcc tggctgaccg
      421 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      481 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      541 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      601 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      661 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      721 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      781 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      841 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      901 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      961 gcttacatgg gtaccactct tgacgacacg gcttaccggt accgcaccag tgtcccgggg
     1021 gacgccgagg ccatcgaggc actggatggg tccttcacca ccgac
//
