LOCUS       sequence-149824-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Logan M, Tabin C
  TITLE     Targeted gene misexpression in chick limb buds using avian 
            replication-competent retroviruses.
  JOURNAL   Methods. 1998 Apr . 14(4):407-20.
  PUBMED    9608511
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            1998 Apr . 14(4):407-20."
COMMENT     Sequence Label: pSLAX13 Flag His (CT#245)
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..85
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(133..149)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(134..150)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(178..197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(179..197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(204..221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(204..220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      362..817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(449..468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     659..680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        843..947
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccaggcggt agctgggacg tgcagccgac caccatggcc atgattacga attccgctag
       61 cgactacaag gacgacgatg acaagggatc tcaccaccac catcaccatg gatcctaact
      121 gcagtctaga atcgataccg tcgacctcga tcgagggggg gcccggtacc caattcgccc
      181 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      361 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      421 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      481 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      541 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      601 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      661 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      721 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      781 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
      841 tgcgcggaac ccctatttgt ttatttttct aaaatacatt caaatatgta tccgctcatg
      901 agacaataac cctgataaat gcttcataat attgaaaaag gaagagtatg agtattcaac
      961 atttccgtgt cgccc
//
