LOCUS       sequence-149829-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to drive expression in zebrafish astrocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p5E-gfap
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..190)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        191..209
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     246..262
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     246..262
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgcggccgcc ctgcagctct agagctcgaa ttctacaggt cactaatacc atctaagtag
       61 ttggttcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      121 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aacttttttg
      181 tacaaacttg aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
      241 gccgctctag aactagtgga tcccgggccc gcggtaccgt cgacgaggaa cgctgggact
      301 ccatggtgga ggagaatgag atcgagtgcg ggtgaactct gggtttttat agagttgatg
      361 tggggggtga aagagagagg gggggtctga tggggagggc aaaggccgag ggggtgtttg
      421 tgagcccgca attgtttgtg cttgtccagc cagtctcctg actgcctctt ttcacagtga
      481 gccagagaaa atcctattca taccctgctg gaacacagaa atagagagac tgagatggca
      541 tcagcactga tattggactt taattctctc ttacctcatg ctaaagagtt ggtttgcaga
      601 tcttctgagt tactccggag ttgctctgct ttctcagtca cagttttgtt ctgtctggga
      661 gtttaatggt ttcagggaac acaggaggtg attacgtaat tcttcattat taaaagaaac
      721 tggcgctccc tcttttgtgc tgatctgaga acatcacaca catgctcttc agcagcactg
      781 atttatatcg gtttagcaga aaaatcatag gaattgattt gtttacttga actcatgctg
      841 gttaaatgtt aaaggggtta ttcatccaat aaacattttg cttgtgtgct ccatatctga
      901 gcacaatttt aacatgc
//
