LOCUS       sequence-149830-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c2-HA3
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      567..764
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(824..841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag
       61 gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc
      121 ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa
      181 tcggacgacg aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg
      241 tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc
      301 cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc
      361 gcgttgatgg ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg
      421 gaggtcacca acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc
      481 tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac
      541 atgagcatgc cctgccccta atcagtactg acaataaaaa gattcttgtt ttcaagaact
      601 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
      661 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
      721 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
      781 aacgccgcca tccagtgtta aacgagctcg aattctaggc ttcctcgctc actgactcgc
      841 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      901 tatccacaga atcaggggat aacgca
//
