LOCUS       sequence-149837-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c2-PK3
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..85
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             89..130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             131..172
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      202..389
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(257..281)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     enhancer        414..717
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        718..921
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     872..892
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     966..985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        966..984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcggtaagc ctattcctaa
       61 ccctcttttg ggtctcgatt ctacaggtgg aaaaccaatc cccaacccac tcctcggcct
      121 tgactcaact ggcaaaccca taccaaatcc tttacttgga ttagatagta cctctagatg
      181 aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
      241 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
      301 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
      361 gtcgctctta ttgaccacac ctctaccggc agatccgcta ggagatctcg cgacgttaca
      421 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      481 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      541 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      601 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      661 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      721 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      781 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      841 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      901 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
      961 gaaattaata cgactcacta tagggagacc caagcttaca tgggtaccac tcttgacgac
     1021 acggcttacc ggtaccgcac cagtgtcccg ggggacgccg aggccatc
//
