LOCUS       sequence-149854-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b2-Myc13
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..549
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      612..799
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(667..691)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgctagcg cgattaacgg tgaacaaaag
       61 ctaatctccg aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac
      121 ggactcgacg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
      181 atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga
      241 ctcgacggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc
      301 tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc
      361 gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
      421 gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac
      481 ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag
      541 gaagacttga acggtgaaca aaaattaatc aatcagtaca agtctagatg aggcgcgcca
      601 cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
      661 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
      721 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta
      781 ttgaccacac ctctaccggc agatccgcta ggagatctgt ttagcttgcc tcgtccccgc
      841 cgggtcaccc ggtcagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
      901 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
      961 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     1021 cgctgcggac ctgcgagcag ggaaacg
//
