LOCUS       sequence-149866-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b2-HA3
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             74..100
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      166..353
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(221..245)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        412..755
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taacgctagc atgtacccat acgatgttcc
       61 tgactatgcg ggctacccgt atgacgtgcc ggactatgca ggagcctatc cttatgacgt
      121 tccagattac gctggttcta gatgaggcgc gccacttcta aataagcgaa tttcttatga
      181 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
      241 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
      301 ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc
      361 gctaggagat ctgtttagct tgcctcgtcc ccgccgggtc acccggtcag cgacatggag
      421 gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
      481 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
      541 ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc ggacctgcga gcagggaaac
      601 gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa
      661 ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga
      721 tacagttctc acatcacatc cgaacataaa caaccatggg taaaaagcct gaactcaccg
      781 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
      841 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
      901 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
      961 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     1021 cctattgcat ctcccgc
//
